Sumo Antibody | ProSci 3969 product information

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product summary

company name :

ProSci

product type :

antibody

product name :

Sumo Antibody

catalog :

3969

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

product information

Catalog Number :

3969

Product Name :

Sumo Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

Sumo Antibody: The sumo family of proteins is related both structurally and functionally to ubiquitin in that they are post-translationally attached to the e-amino group of a lysine residue of the substrate protein. This sumoylation plays a number of roles in DNA replication and repair, protein targeting to various subnuclear structures, and the regulation of numerous cellular processes including the inflammatory response in mammalian cells. Sumo was initially identified as a covalent modification of RanGAP1 in studies on nuclear import in mammalian cells. More recently, sumo has been shown to be involved in the regulation of transcription factors, possibly by enhancing their interactions with co-repressors. Sumo is also thought to play some role in the modulation of ubiquitin-mediated degradation of proteins by acting as an inhibitor. At least four different isoforms of sumo are known to exist; Sumo antibody will only recognize isoform 1.

ALTnames :

Sumo Antibody: DAP1, GMP1, PIC1, SMT3, UBL1, OFC10, SENP2, SMT3C, SMT3H3, OK/SW-cl.43, Small ubiquitin-related modifier 1, GAP-modifying protein 1, SUMO-1

Immunogen :

Sumo antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human sumo.

Purity :

Sumo Antibody is affinity chromatography purified via peptide column.

Buffer :

Sumo Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

Sumo antibody can be used for detection of sumo by Western blot at 0.5 - 2 g/mL. Antibody can also be used for immunohistochemistry starting at 5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

Sumo antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Dohmen RJ. Sumo protein modification. Biochim. Biophys. Acta 2004; 1695:113-31.

General References :

Matunis MJ, Coutavas E, and Blobel G. A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex. J. Cell Biol. 1996; 135:1457-70.

General References :

Gill G. Something about SUMO inhibits transcription. Curr. Opin. Genet. Dev. 2005; 15:536-41.

General References :

Desterro JM, Rodriguez MS, and Hay RT. SUMO-1 modification of IkappaBalpha inhibits NFkappaB activation. Mol. Cell 1998; 2:233-9.