TIP47 Antibody | ProSci 3883 product information

product summary

company name :

ProSci

product type :

antibody

product name :

TIP47 Antibody

catalog :

3883

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry

more info or order :

ProSci product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:1,000	Macpherson R, Castelli L, Miotto P, Frendo-Cumbo S, Milburn A, Roy B, et al. A maternal high fat diet has long-lasting effects on skeletal muscle lipid and PLIN protein content in rat offspring at young adulthood. Lipids. 2015;50:205-17 pubmed publisher
western blot; human	Peters S, Samjoo I, Devries M, Stevic I, Robertshaw H, Tarnopolsky M. Perilipin family (PLIN) proteins in human skeletal muscle: the effect of sex, obesity, and endurance training. Appl Physiol Nutr Metab. 2012;37:724-35 pubmed publisher
western blot; mouse	Macpherson R, Vandenboom R, Roy B, Peters S. Skeletal muscle PLIN3 and PLIN5 are serine phosphorylated at rest and following lipolysis during adrenergic or contractile stimulation. Physiol Rep. 2013;1:e00084 pubmed publisher
western blot; rat; 1:1000; fig 3	Ramos S, Macpherson R, Turnbull P, Bott K, LeBlanc P, Ward W, et al. Higher PLIN5 but not PLIN3 content in isolated skeletal muscle mitochondria following acute in vivo contraction in rat hindlimb. Physiol Rep. 2014;2: pubmed publisher

product information

Catalog Number :

3883

Product Name :

TIP47 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

TIP47 Antibody: Tail-interacting protein (TIP47) is a cytosolic protein essential for the transport of mannose-6-phosphate receptors (MPRs) from endosomes to the trans-Golgi compartments in cells. TIP47 is recruited from the cytoplasm to the late endosomal surface by the Ras-associated protein Rab9 GTPase, enabling it to bind more efficiently to MPR cytoplasmic domains. Recently, it was shown that TIP47 regulates the expression of Rab9, as expression of siRNA to TIP47 in transfected cells dramatically decreased the half-life of the Rab9 protein in addition to stabilizing the subcellular localization of Rab9. At least two isoforms of TIP47 are known to exist.

ALTnames :

TIP47 Antibody: PP17, TIP47, M6PRBP1, Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein

Immunogen :

TIP47 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human TIP47.

Purity :

TIP47 Antibody is affinity chromatography purified via peptide column.

Buffer :

TIP47 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC

Application Summary :

TIP47 antibody can be used for the detection of TIP47 by Western blot at 0.5 - 1 g/mL. Antibody can also be used for immunohistochemistry starting at 2 g/mL.

Storage :

TIP47 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Barbero P, Bittova L, and Pfeffer SR. Visualization of Rab9-mediated vesicle transport from endosomes to the trans Golgi in living cells. J. Cell Biol. 2002; 156:511-8.

General References :

Carroll KS, Hanna J, Simon I, et al. Role of the Rab9 GTPase in facilitating receptor recruitment by TIP47. Science 2001; 292:1373-7.

General References :

Aivazian D, Serrano RL, and Pfeffer S. TIP47 is a key effector for Rab9 localization. J. Cell Biol. 2006; 173:917-26.

General References :

Ganley IG, Carroll K, Bittova L, et al. Rab9 GTPase regulates late endosome size and requires effector interaction for its stability. Mol. Biol. Cell 2004; 15:5420-30.

more info or order :

ProSci product webpage