IL-6 Antibody | ProSci 38-201 product information

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product summary

company name :

ProSci

product type :

antibody

product name :

IL-6 Antibody

catalog :

38-201

quantity :

0.1 mg

price :

315 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, neutralization

product information

Catalog Number :

38-201

Product Name :

IL-6 Antibody

Host Species :

Goat

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.1 mg

List Price :

315 USD

CrossReactivity :

Human

Background :

Interleukin 6 (IL-6) is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL-6 has also been implicated in regulating adipose mass.

ALTnames :

HGF, HSF, BSF2, IL-6, IFNB2, Interleukin-6, B-cell stimulatory factor 2

Immunogen :

Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-6. Human IL-6 specific antibody was purified by affinity chromatography employing immobilized hIL-6 matrix.

Uses :

E, WB, Neut

Application Summary :

Neutralization:. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-6 (5 ng/mL), a concentration of 0.05 - 0.08 g/mL of this antibody is required. ELISA:. Indirect:. To detect hIL-6 by indirect ELISA (using 100 L/well antibody solution) a concentration of 0.5 - 2.0 g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6. Sandwich. To detect hIL-6 by sandwich ELISA (using 100 L/well antibody solution) a concentration of 0.5 - 2.0 g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human IL-6 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6. Western Blot:. To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Storage :

IL-6 antibody is stable for at least 2 years from date of receipt at -20 C. The reconstituted antibody is stable for at least two weeks at 2-8 C. Frozen aliquots are stable for at least 6 months when stored at -20 C. Avoid repeated freeze-thaw cycles.