BMI-1 Antibody | ProSci 3755 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

BMI-1 Antibody

catalog :

3755

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

product information

Catalog Number :

3755

Product Name :

BMI-1 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

BMI-1 Antibody: The transcriptional repressor BMI-1 was first identified as a proto-oncogene frequently activated by Moloney murine leukemia proviral insertions in mice and cooperating with c-myc in the generation of mouse lymphomas. BMI-1 is involved in segment specification, cell growth and maintenance, transcriptional regulation, and chromatin modification. A major target of BMI-1 is the ink4a locus which encodes tumor suppressor proteins p16 and p19Arf, which are important in tumor progression and thought to be critical in cell proliferation and senescence. Recent studies have also shown that BMI-1 is required for the maintenance of adult normal and leukemic stem cells, suggesting that BMI-1 could an attractive therapeutic target for stem cell proliferation and renewal as well as for anti-cancer strategies.

ALTnames :

BMI-1 Antibody: PCGF4, RNF51, FLVI2/BMI1, PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4

Immunogen :

BMI-1 antibody was raised against a peptide corresponding to 15 amino acids near the center of human BMI-1.

Purity :

BMI-1 Antibody is affinity chromatography purified via peptide column.

Buffer :

BMI-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, ICC, IF

Application Summary :

BMI-1 antibody can be used for detection of BMI-1 by Western blot at 0.5 to 2 g/mL. Antibody can also be used for immunocytochemistry starting at 10 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

BMI-1 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Alkema MJ, Wiegant J, Raap AK, et al. Characterization and chromosomal localization of the human proto-oncogene BMI-1. Hum. Mol. Genet. 1993; 2:1597-603.

General References :

Jacobs JJ, Kieboom K, Marino S, et al. The oncogene and polycomb-group gene bmi-1 regulates cell proliferation and senescence through the ink4a locus. Nature 1999; 397:164-8.

General References :

Lessard J and Sauvageau G. BMI-1 determines the proliferative capacity of normal and leukaemic stem cells. Nature 2003; 255-60.