ProSci

antibody

TLR9 Antibody

3739

0.02 mg, 0.1 mg

79, 315 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunohistochemistry, immunocytochemistry

3739

TLR9 Antibody

Rabbit

Polyclonal

Unconjugated

Primary Antibody

0.02 mg, 0.1 mg

79, 315 USD

IgG

Human, Mouse, Rat

TLR9 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.

TLR9 Antibody: CD289, Toll-like receptor 9

TLR9 antibody was raised against a peptide corresponding to 15 amino acids near the center of human TLR9.

At least two isoforms of TLR9 are known to exist; this antibody will detect both isoforms. TLR9 antibody is predicted to not cross-react with other TLR proteins.

TLR9 Antibody is affinity chromatography purified via peptide column.

TLR9 Antibody is supplied in PBS containing 0.02% sodium azide.

1 mg/mL

E, WB, ICC, IF

TLR9 antibody can be used for detection of TLR9 by Western blot at 0.5 to 2 g/mL. Antibody can also be used for immunocytochemistry starting at 2 g/mL. For immunofluorescence start at 20 g/mL.

TLR9 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol.2003; 21:335-76.

Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol.2002; 20:197-216.

Wagner H. The immunobiology of the TLR9 subfamily. Trends Immunol.2004; 381-6

Nishiya T and DeFranco AL. Ligand-regulated chimeric receptor approach reveals distinctive subcellular localization and signaling properties of the Toll-like receptors. J. Biol. Chem.2004; 279:19008-17.