XBP-1 Antibody | ProSci 3685 product information

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product summary

company name :

ProSci

product type :

antibody

product name :

XBP-1 Antibody

catalog :

3685

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry

product information

Catalog Number :

3685

Product Name :

XBP-1 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

XBP-1 Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.

ALTnames :

XBP-1 Antibody: XBP2, TREB5, XBP-1, XBP2, X-box-binding protein 1, Tax-responsive element-binding protein 5

Immunogen :

XBP-1 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human XBP-1.

Purity :

XBP-1 Antibody is affinity chromatography purified via peptide column.

Buffer :

XBP-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, ICC

Application Summary :

XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 0.5 - 2 g/mL. Antibody can also be used for immunocytochemistry starting at 2 g/mL.

Storage :

XBP-1 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.

General References :

Calfon M, Zeng H, Urano F, et al. IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Nature 2002; 415:92-6.

General References :

Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.

General References :

Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.