PIST Antibody | ProSci 3631 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

PIST Antibody

catalog :

3631

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

product information

Catalog Number :

3631

Product Name :

PIST Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

PIST Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.

ALTnames :

PIST Antibody: CAL, FIG, PIST, GOPC1, dJ94G16.2, CAL, Golgi-associated PDZ and coiled-coil motif-containing protein, CFTR-associated ligand

Immunogen :

PIST antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human PIST.

Purity :

PIST Antibody is affinity chromatography purified via peptide column.

Buffer :

PIST Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

PIST antibody can be used for the detection of PIST by Western blot at 1 - 4 g/mL. Antibody can also be used for immunohistochemistry starting at 1 g/mL. For immunofluorescence start at 10 g/mL.

Storage :

PIST antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.

General References :

Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells.Carcinogenesis 1993; 14:2501-5.

General References :

Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.

General References :

Yu Z, Horton A, Bravin M, et al. A novel protein complex linking the 2 glutamate receptor and autophagy: implications for neurodegeneration in Lurcher mice. Neuron 2002; 35:921-33.