APG7 Antibody | ProSci 3617 product information

product summary

company name :

ProSci

product type :

antibody

product name :

APG7 Antibody

catalog :

3617

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry

more info or order :

ProSci product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; human	Yang N, Tan S, Ng S, Shi Y, Zhou J, Tan K, et al. Artesunate induces cell death in human cancer cells via enhancing lysosomal function and lysosomal degradation of ferritin. J Biol Chem. 2014;289:33425-41 pubmed publisher
western blot; human; 1:500	Vandergaast R, Fredericksen B. West Nile virus (WNV) replication is independent of autophagy in mammalian cells. PLoS ONE. 2012;7:e45800 pubmed publisher
western blot; mouse	Zhang J, Kundu M, Ney P. Mitophagy in mammalian cells: the reticulocyte model. Methods Enzymol. 2009;452:227-45 pubmed publisher
	Ambjørn M, Ejlerskov P, Liu Y, Lees M, Jaattela M, Issazadeh Navikas S. IFNB1/interferon-?-induced autophagy in MCF-7 breast cancer cells counteracts its proapoptotic function. Autophagy. 2013;9:287-302 pubmed
	Xue L, Chiu S, Oleinick N. Atg7 deficiency increases resistance of MCF-7 human breast cancer cells to photodynamic therapy. Autophagy. 2010;6:248-55 pubmed

product information

Catalog Number :

3617

Product Name :

APG7 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse

Background :

APG7 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.

ALTnames :

APG7 Antibody: GSA7, APG7L, APG7-LIKE, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, APG7-like

Immunogen :

APG7 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human APG7.

Purity :

APG7 Antibody is affinity chromatography purified via peptide column.

Buffer :

APG7 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, ICC, IF

Application Summary :

APG7 antibody can be used for the detection of APG7 by Western blot at 1 - 4 g/mL. Antibody can also be used for immunocytochemistry starting at 10 g/mL. For immunofluorescence start at 10 g/mL.

Storage :

APG7 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.

General References :

Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.

General References :

Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.

General References :

Mizushima N, Noda T, Yoshimori T, et al. A protein conjugation system essential for autophagy. Nature 1998; 395:395-8.

more info or order :

ProSci product webpage