4E-BP1 Antibody | ProSci 3513 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

4E-BP1 Antibody

catalog :

3513

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry

product information

Catalog Number :

3513

Product Name :

4E-BP1 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse

Background :

4E-BP1 Antibody: The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms. One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5'-UTRs. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferationl, at least in part by inhibiting the activity of TOR towards 4E-BP1.

ALTnames :

4E-BP1 Antibody: BP-1, 4EBP1, 4E-BP1, PHAS-I, Eukaryotic translation initiation factor 4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1

Immunogen :

4E-BP1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4E-BP1.

Purity :

4E-BP1 Antibody is affinity chromatography purified via peptide column.

Buffer :

4E-BP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, ICC

Application Summary :

4E-BP1 antibody can be used for the detection of 4E-BP1 by Western blot at 2.5 - 10 g/mL. Antibody can also be used for immunocytochemistry starting at 2 g/mL.

Storage :

4E-BP1 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Shah OJ, Anthony JC, Kimball SR, et al. 4E-BP1 and S6K1: translational integration sites for nutritional and hormonal information in muscle. Am. J. Physiol. Endocrinol. Metab. 2000; 279:E715-29.

General References :

Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80.