PIKE Antibody | ProSci 3321 product information

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product summary

company name :

ProSci

product type :

antibody

product name :

PIKE Antibody

catalog :

3321

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry

product information

Catalog Number :

3321

Product Name :

PIKE Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse

Background :

PIKE Antibody: Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes. In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.

ALTnames :

PIKE Antibody: PIKE, GGAP2, CENTG1, KIAA0167, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2, Centaurin-gamma-1, AGAP-2

Immunogen :

PIKE antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human PIKE.

Specificity :

Anti-PIKE will detect both PIKE-L and PIKE-A isoforms.

Purity :

PIKE Antibody is Ion exchange chromatography purified.

Buffer :

PIKE Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC

Application Summary :

PIKE antibody can be used for detection of PIKE by Western blot at 0.5 to 2 g/mL. Antibody can also be used for immunohistochemistry starting at 10 g/mL.

Storage :

PIKE antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Ye K, Hurt KJ, Wu FY, et al. Pike. A nuclear gtpase that enhances PI3kinase activity and is regulated by protein 4.1N. Cell 2000; 103:919-30.

General References :

Jackson TR, Kearns BG, and Thiebert AB. Cytohesins and centaurins: mediators of PI3-kinase-regulated Arf signaling. Trends Biochem. Sci. 2000; 25:489-95.

General References :

Rong R, Ahn JY, Huang H, et al. PI3 kinase enhancer-Homer complex couples mGluRI to PI3 kinase, preventing neuronal apoptosis. Nat. Neurosci. 2003; 6:1153-61.

General References :

Ahn J-Y, Rong R, Kroll TG, et al. PIKE (Phosphotidylinositol 3-kinase enhancer)-A GTPase stimulates Akt activity and mediates cellular invasion. J. Biol. Chem. 2004; 279:16441-51.