DAD1 Antibody | ProSci 3313 product information

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product summary

company name :

ProSci

product type :

antibody

product name :

DAD1 Antibody

catalog :

3313

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry

product information

Catalog Number :

3313

Product Name :

DAD1 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse

Background :

DAD1 Antibody: Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.

ALTnames :

DAD1 Antibody: OST2, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Defender against cell death 1, Oligosaccharyl transferase subunit DAD1

Immunogen :

DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1.

Purity :

DAD1 Antibody is Ion exchange chromatography purified.

Buffer :

DAD1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, ICC, IF

Application Summary :

DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 g/mL. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 10 g/mL. For immunofluorescence start at 10 g/mL.

Storage :

DAD1 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Nakashima T, Sekiguchi T, Kuraoka A, et al. Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells. Mol. Cell Biol. 1993; 13:6367-74.

General References :

Stellar H. Mechanisms and genes of cellular suicide. Science 1995; 267:1445-9.

General References :

Gilmore R and Kelleher DJ. DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase. Proc. Natl. Acad. Sci. USA 1997; 94:4994-9.

General References :

Sanjay A, Fu J, and Kreibich G. DAD1 is required for the function and the structural integrity of the oligosaccharyltransferase complex. J. Biol. Chem. 1998; 273:26094-9.