ProSci

antibody

XAF-1 Antibody

3207

0.02 mg, 0.1 mg

79, 315 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunohistochemistry, immunocytochemistry

3207

XAF-1 Antibody

Rabbit

Polyclonal

Unconjugated

Primary Antibody

0.02 mg, 0.1 mg

79, 315 USD

IgG

Human, Mouse

XAF-1 Antibody: XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.

XAF-1 Antibody: BIRC4BP, XIAPAF1, HSXIAPAF1, BIRC4BP, XIAP-associated factor 1, BIRC4-binding protein

XAF-1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1.

XAF-1 Antibody is affinity chromatography purified via peptide column.

XAF-1 Antibody is supplied in PBS containing 0.02% sodium azide.

1 mg/mL

E, WB, IHC, IF

XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 0.5 to 2 g/mL. A 32 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 g/mL. For immunofluorescence start at 10 g/mL.

XAF-1 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Liston P, Fong W, Kelly NL, et al. Identification of XAF1 as an antagonist of XIAP anticaspase activity. Nature Cell Biol. 2001;3:128-33.

Deveraux QL, Takahashi R, Savesan GS, and Reed JC. X-linked IAP is a direct inhibitor of cell-death proteases. Nature 1997;388:300-4.

Fong WG, Liston P, Rajcan-Separovic E, et al. Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines. Genomics 2000;70:113-122.

Leaman DW, Chawla-Sarkar M, Vyas K, et al. Identification of X-linked inhibitor of apoptosis associated factor-1 as an interferon-stimulated gene that augments TRAIL/Apo2L-induced apoptosis. J. Biol. Chem. 2002;277:28504-11.