P16 Antibody [1E12E10] | ProSci 32-203 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

P16 Antibody [1E12E10]

catalog :

32-203

quantity :

0.1 mg

price :

335 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1E12E10

The same clone is also sold as:

ProMab: 20130
LifeSpan Biosciences: LS-B5261
Abnova: MAB10402, MAB16146
MyBioSource: MBS532973
Invitrogen: MA5-17142
Biorbyt: orb97580

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry

product information

Catalog Number :

32-203

Product Name :

P16 Antibody [1E12E10]

Host Species :

Mouse

Clonality :

Monoclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.1 mg

List Price :

335 USD

Clone :

1E12E10

Isotype :

IgG1

CrossReactivity :

Human, Mouse, Rat

Background :

The progression of cells through the cell cycle is regulated by a family of protein kinases known as cyclin-dependent kinases (Cdks). The sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. The cyclins function as differentially expressed positive regulators of Cdks. Negative regulators of the cycle include the p53-inducible 21 kDa WAF1/Cip1 protein designated p21, Kip1 p27 and p16. The complexes formed by Cdk4 and the D-type cyclins have been strongly implicated in the control of cell proliferation during the G1 phase. It has recently been shown that p16 binds to Cdk4 and inhibits the catalytic activity of the Cdk4/cyclin D complex. Moreover, the gene encoding p16 exhibits a high frequency of homozygous deletions and point mutations in established human tumor cell lines.

ALTnames :

ARF, MLM, P14, P16, P19, CMM2, INK4, MTS1, TP16, CDK4I, CDKN2, INK4A, MTS-1, P14ARF, P19ARF, P16INK4, P16INK4A, P16-INK4A

Immunogen :

Ni-NTA purified truncated recombinant P16 expressed in E. Coli strain BL21 (DE3).

Buffer :

Ascitic fluid containing 0.03% sodium azide.

Uses :

E, WB, IHC

Application Summary :

Western Blot:1:500 - 1:2,000 . IHC(P):1:500 - 1:2,000. ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.

Storage :

P16 (Mouse and Human) monoclonal antibody can be stored at -20 C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Hunter, T. 1993. Cell 75: 839-841.

General References :

Sherr, C.J. 1993. Cell 73: 1059-1065.

General References :

El-Deiry, W.S., et al. 1993. Cell 75: 817-825.