P16 Antibody [5A8A4 / 3G8D12] | ProSci 32-172 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

P16 Antibody [5A8A4 / 3G8D12]

catalog :

32-172

quantity :

0.1 mL

price :

285 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5A8A4 / 3G8D12

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

product information

Catalog Number :

32-172

Product Name :

P16 Antibody [5A8A4 / 3G8D12]

Host Species :

Mouse

Clonality :

Monoclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.1 mL

List Price :

285 USD

Clone :

5A8A4 / 3G8D12

CrossReactivity :

Human

Background :

p16 (cyclin-dependent kinase inhibitor 2A, INK4a) is a tumor suppressor protein. It is a specific inhibitor of Cdk 4 / Cdk 6, and a tumor suppressor involved in the pathogenesis of a variety of malignancies. Recent analyses of the p16 INK4a gene revealed homozygous deletions, nonsense, missense, or frameshift mutations in several human cancers. Although the frequency of p16 INK4a abnormalities is higher in tumor derived cell lines than in unselected primary tumors, significant subsets of clinical cases with aberrant p16 INK4a gene have been reported among melanomas, gliomas, esophageal, pancreatic, lung, and urinary bladder carcinomas, and some types of leukemia.

ALTnames :

ARF, MLM, P14, P16, P19, CMM2, INK4, MTS1, TP16, CDK4I, CDKN2, INK4A, MTS-1, P14ARF, P19ARF, P16INK4, P16INK4A, P16-INK4A

Immunogen :

Ni-NTA purified truncated recombinant P16 expressed in E. Coli strain BL21 (DE3).

Buffer :

Purified antibody in PBS containing 0.03% sodium azide

Uses :

E, WB, IHC

Application Summary :

Western Blot:1:500 - 1:2,000 . IHC(P):1:200 - 1:1,000. ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.

Storage :

Human P16 monoclonal antibody can be stored at -20 C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Bai, F. et al. Mol. Cell. Biol.2003 23, 1269-1277.

General References :

Lowe, S.W. and Sherr, C.J. Curr. Opin. Genet.2003 Dev.13, 77-83.

General References :

Sherr, C.J. Nat. Rev. Mol. Cell Biol.2001 2, 731-737.