CARMA1 Antibody | ProSci 3189 product information

product summary

company name :

ProSci

product type :

antibody

product name :

CARMA1 Antibody

catalog :

3189

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

ProSci product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human	Chan W, Schaffer T, Pomerantz J. A quantitative signaling screen identifies CARD11 mutations in the CARD and LATCH domains that induce Bcl10 ubiquitination and human lymphoma cell survival. Mol Cell Biol. 2013;33:429-43 pubmed publisher
	Pedersen S, Chan W, Jattani R, Mackie d, Pomerantz J. Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181. Mol Cell Biol. 2015;36:794-808 pubmed publisher
	Shin H, Tilahun M, Cho O, Chandiran K, Kuksin C, Keerthivasan S, et al. NOTCH1 Can Initiate NF-κB Activation via Cytosolic Interactions with Components of the T Cell Signalosome. Front Immunol. 2014;5:249 pubmed publisher

product information

Catalog Number :

3189

Product Name :

CARMA1 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse

Background :

CARMA1 Antibody: CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. Both CARMA1 and CARMA3 associate with NEMO, the regulatory subunit of the I K complex, thereby regulating activation of the NF- B transcription factor. Also, gene inactivation studies showed a complete block in T and B cell immunity as well as an impaired response to LPS, indicating that CARMA1 is a critical regulator in both the adaptive and innate immune systems.

ALTnames :

CARMA1 Antibody: PPBL, BENTA, BIMP3, IMD11, CARMA1, Caspase recruitment domain-containing protein 11, CARD-containing MAGUK protein 1, Carma 1

Immunogen :

CARMA1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARMA1.

Purity :

CARMA1 Antibody is affinity chromatography purified via peptide column.

Buffer :

CARMA1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

CARMA1 antibody can be used for detection of CARMA1 by Western blot at 0.5 to 2 g/mL. A band at approximately 125 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 10 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

CARMA1 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Fanning AS and Anderson JM. Protein modules as organizers of membrane structure.Curr. Opin. Cell Biol. 1999; 11:432-9.

General References :

Gaide O, Martinon F, Michau O, et al. Carma1, 1 CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF- B activation. FEBS Lett. 2001; 496:121-7.

General References :

Bertin J, Wang L, Guo Y, et al. CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF- B. J. Biol. Chem. 2001; 276:11877-82.

General References :

McAllister-Lucas LM, Inohara N, Lucas PC, et al. Bimp1, a MAGUK family member linking protein kinase C activation to Bcl10-mediated NF- B induction. J. Biol. Chem. 2001; 276:30589-97.

more info or order :

ProSci product webpage