IRAK-4 Antibody | ProSci 3125 product information

product summary

company name :

ProSci

product type :

antibody

product name :

IRAK-4 Antibody

catalog :

3125

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

ProSci product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human	Heinz L, Rebsamen M, Rossi D, Staehli F, Schroder K, Quadroni M, et al. The death domain-containing protein Unc5CL is a novel MyD88-independent activator of the pro-inflammatory IRAK signaling cascade. Cell Death Differ. 2012;19:722-31 pubmed publisher

product information

Catalog Number :

3125

Product Name :

IRAK-4 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human

Background :

IRAK-4 Antibody: Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR). IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF- B and MAP kinase (MAPK) by IL-1R/TLR. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4. Overexpression of IRAK-4 activates NF- B and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.

ALTnames :

IRAK-4 Antibody: IPD1, REN64, IRAK-4, NY-REN-64, Interleukin-1 receptor-associated kinase 4, Renal carcinoma antigen NY-REN-64

Immunogen :

IRAK-4 antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK-4.

Specificity :

IRAK-4 antibody is predicted to not cross-react with other members of the IRAK protein family.

Purity :

IRAK-4 Antibody is affinity chromatography purified via peptide column.

Buffer :

IRAK-4 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, ICC, IF

Application Summary :

IRAK-4 antibody can be used for the detection of IRAK-4 by Western blot at 1 to 4 g/mL. Antibody can also be used for immunocytochemistry starting at 10 g/mL. For immunofluorescence start at 10 g/mL.

Storage :

IRAK-4 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Cao Z, Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31.

General References :

Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5

General References :

Wesche H, Gao X, Li X, et al. IRAK-M is a novel member of the Pelle/interleukin-1 receptor-associated kinase (IRAK) family. J. Biol. Chem. 1999; 274:19403-10.

General References :

Li S, Strelow A, Fontana EJ, et al. IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase. Proc. Natl. Acad. Sci. USA 2002; 99:5567-72.

more info or order :

ProSci product webpage