Ipaf Antibody | ProSci 3107 product information

product summary

company name :

ProSci

product type :

antibody

product name :

Ipaf Antibody

catalog :

3107

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section

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citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; loading ...; fig 2b	Janowski A, Colegio O, Hornick E, McNiff J, Martin M, Badovinac V, et al. NLRC4 suppresses melanoma tumor progression independently of inflammasome activation.. J Clin Invest. 2016;126:3917-3928 pubmed publisher
western blot; human; fig s1b	Zhou R, Tardivel A, Thorens B, Choi I, Tschopp J. Thioredoxin-interacting protein links oxidative stress to inflammasome activation. Nat Immunol. 2010;11:136-40 pubmed publisher

product information

Catalog Number :

3107

Product Name :

Ipaf Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human

Background :

Ipaf Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adaptor molecules and proteases including several members of the caspase family. Another family of proteins that functions as a critical regulator of apoptosis and NF signaling pathways is the CED-4/Apaf-1 (apoptosis protein activating factor-1) protein family. Ipaf (ICE protease activating factor) is a CED-4/Apaf-1 family member that activates caspase-1/ICE and can induce apoptosis in human cells in a caspase-1 dependent manner. Ipaf and caspase-1 are thought to interact with each other through the association of the Ipaf amino-terminal CARD (caspase recruitment domain) and amino-terminal CARD of caspase-1.

ALTnames :

Ipaf Antibody: CLAN, IPAF, CLAN1, CLANA, CLANB, CLANC, CLAND, CARD12, CLR2.1, CLAN, UNQ6189/PRO20215, NLR family CARD domain-containing protein 4, CARD, LRR, and NACHT-containing protein, Clan protein

Immunogen :

Ipaf antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of human Ipaf.

Purity :

Ipaf Antibody is affinity chromatography purified via peptide column.

Buffer :

Ipaf Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, ICC, IF

Application Summary :

Ipaf polyclonal antibody can be used for the detection of Ipaf by Western blot at 1 and 2 g/mL. A 110 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 g/mL. For immunofluorescence start at 10 g/mL.

Storage :

Ipaf antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997; 91:479-89.

General References :

Poyet J-L, Srinivaula SM, Tnani M, et al. Identification of Ipaf, a human caspase-1-activating protein related to Apaf-1. J. Biol. Chem. 2001; 276:28309-13.

General References :

Geddes BJ, Wang L, Huang WJ, et al. Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis. Biochem. Biophys. Res. Commun. 2001; 284:77-82.

more info or order :

ProSci product webpage