PCNA Antibody | ProSci 31-033 product information

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product summary

company name :

ProSci

product type :

antibody

product name :

PCNA Antibody

catalog :

31-033

quantity :

100 ug

price :

250 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog

application :

western blot, ELISA, immunohistochemistry

product information

Catalog Number :

31-033

Product Name :

PCNA Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

100 ug

List Price :

250 USD

CrossReactivity :

Human, Mouse, Rat, Dog

Background :

PCNA is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

ALTnames :

PCNA, MGC8367,

Immunogen :

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCNA.

Purity :

Antibody is purified by protein A chromatography method.

Buffer :

Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration :

1 mg/ml

Uses :

E, WB, IHC

Application Summary :

PCNA antibody can be used for detection of PCNA by ELISA at 1:62500. PCNA antibody can be used for detection of PCNA by western blot at 1.25 g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Storage :

For short periods of storage (days) store at 4 C. For longer periods of storage, store PCNA antibody at -20 C. As with any antibody avoid repeat freeze-thaw cycles.

General References :

Senga, T., (2006) J. Biol. Chem. 281 (10), 6246-6252.