PUMA Antibody | ProSci 3043 product information

product summary

company name :

ProSci

product type :

antibody

product name :

PUMA Antibody

catalog :

3043

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

ProSci product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; human; fig 2 western blot; human; fig 1	Xie W, Zhang L, Jiao H, Guan L, Zha J, Li X, et al. Chaperone-mediated autophagy prevents apoptosis by degrading BBC3/PUMA. Autophagy. 2015;11:1623-35 pubmed publisher
western blot; human; 1:1000	Sosa Seda I, Mott J, Akazawa Y, Barreyro F, Bronk S, Kaufmann S, et al. Noxa mediates hepatic stellate cell apoptosis by proteasome inhibition. Hepatol Res. 2010;40:701-10 pubmed
western blot; mouse	Ambacher K, Pitzul K, Karajgikar M, Hamilton A, Ferguson S, Cregan S. The JNK- and AKT/GSK3β- signaling pathways converge to regulate Puma induction and neuronal apoptosis induced by trophic factor deprivation. PLoS ONE. 2012;7:e46885 pubmed publisher
western blot; mouse	Lee Y, Moujalled D, Doerflinger M, Gangoda L, Weston R, Rahimi A, et al. CREB-binding protein (CBP) regulates β-adrenoceptor (β-AR)-mediated apoptosis. Cell Death Differ. 2013;20:941-52 pubmed publisher
immunohistochemistry - frozen section; rat; 1:200	Wakabayashi T, Kosaka J, Mori T, Yamada H. Prolonged expression of Puma in cholinergic amacrine cells during the development of rat retina. J Histochem Cytochem. 2012;60:777-88 pubmed
	Sale M, Cook S. The BH3 mimetic ABT-263 synergizes with the MEK1/2 inhibitor selumetinib/AZD6244 to promote BIM-dependent tumour cell death and inhibit acquired resistance. Biochem J. 2013;450:285-94 pubmed publisher
	Wade M, Rodewald L, Espinosa J, Wahl G. BH3 activation blocks Hdmx suppression of apoptosis and cooperates with Nutlin to induce cell death. Cell Cycle. 2008;7:1973-82 pubmed

product information

Catalog Number :

3043

Product Name :

PUMA Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human

Background :

PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

ALTnames :

PUMA Antibody: JFY1, PUMA, JFY-1, Bcl-2-binding component 3

Immunogen :

PUMA antibody was raised against a synthetic peptide corresponding to 14 amino acids near the amino terminus of human PUMA-a. This sequence is identical between a and b forms of the PUMA proteins.

Purity :

PUMA Antibody is affinity chromatography purified via peptide column.

Buffer :

PUMA Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

PUMA antibody can be used for detection of PUMA by Western blot at 2 g/mL. Antibody can also detect PUMA by immunohistochemistry at 10 g/mL. For immunofluorescence start at 10 g/mL.

Storage :

PUMA antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001;7(3):683-94.

General References :

Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001;7(3):673-82.

General References :

Han J, Flemington C, Houghton AB , Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001;98(20):11318-23.

more info or order :

ProSci product webpage