PUMA Antibody | ProSci 3041 product information

product summary

company name :

ProSci

product type :

antibody

product name :

PUMA Antibody

catalog :

3041

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry

more info or order :

ProSci product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 3	O Neill K, Huang K, Zhang J, Chen Y, Luo X. Inactivation of prosurvival Bcl-2 proteins activates Bax/Bak through the outer mitochondrial membrane. Genes Dev. 2016;30:973-88 pubmed publisher
western blot; mouse; fig 5 western blot; rat; fig 2	Sabirzhanov B, Stoica B, Zhao Z, Loane D, Wu J, Dorsey S, et al. miR-711 upregulation induces neuronal cell death after traumatic brain injury. Cell Death Differ. 2016;23:654-68 pubmed publisher
western blot; human western blot; rat western blot; mouse	Sabirzhanov B, Zhao Z, Stoica B, Loane D, Wu J, Borroto C, et al. Downregulation of miR-23a and miR-27a following experimental traumatic brain injury induces neuronal cell death through activation of proapoptotic Bcl-2 proteins. J Neurosci. 2014;34:10055-71 pubmed publisher
western blot; mouse	Brochier C, Dennis G, Rivieccio M, McLaughlin K, Coppola G, Ratan R, et al. Specific acetylation of p53 by HDAC inhibition prevents DNA damage-induced apoptosis in neurons. J Neurosci. 2013;33:8621-32 pubmed publisher
	Lee T, Pelletier J. Dependence of p53-deficient cells on the DHX9 DExH-box helicase. Oncotarget. 2017;8:30908-30921 pubmed publisher
	Weyhenmeyer B, Noonan J, WÃ¼rstle M, Lincoln F, Johnston G, Rehm M, et al. Predicting the cell death responsiveness and sensitization of glioma cells to TRAIL and temozolomide. Oncotarget. 2016;7:61295-61311 pubmed publisher
	Shi R, Peng H, Yuan X, Zhang X, Zhang Y, Fan D, et al. Down-regulation of c-fos by shRNA sensitizes adriamycin-resistant MCF-7/ADR cells to chemotherapeutic agents via P-glycoprotein inhibition and apoptosis augmentation. J Cell Biochem. 2013;114:1890-900 pubmed publisher

product information

Catalog Number :

3041

Product Name :

PUMA Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse

Background :

PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

ALTnames :

PUMA Antibody: JFY1, PUMA, JFY-1, Bcl-2-binding component 3

Immunogen :

PUMA antibody was raised against a synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human PUMA-a. This sequence is identical between a and b forms of the PUMA proteins.

Specificity :

A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-b form.

Purity :

PUMA Antibody is affinity chromatography purified via peptide column.

Buffer :

PUMA Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, ICC, IF

Application Summary :

PUMA antibody can be used for detection of PUMA by Western blot at 2 g/mL. Antibody can also be used for immunocytochemstry at 1 g/mL. For immunofluorescence start at 2 g/mL.

Storage :

PUMA antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001;7(3):683-94.

General References :

Han J, Flemington C, Houghton AB , Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001;98(20):11318-23.

more info or order :

ProSci product webpage