EndoG Antibody | ProSci 3035 product information

product summary

company name :

ProSci

product type :

antibody

product name :

EndoG Antibody

catalog :

3035

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

ProSci product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:500; loading ...; fig 4a western blot; human; 1:500; loading ...; fig 3a	Gole B, Baumann C, Mian E, Ireno C, Wiesmuller L. Endonuclease G initiates DNA rearrangements at the MLL breakpoint cluster upon replication stress. Oncogene. 2015;34:3391-401 pubmed publisher
western blot; human	Wolff S, Erster S, Palacios G, Moll U. p53's mitochondrial translocation and MOMP action is independent of Puma and Bax and severely disrupts mitochondrial membrane integrity. Cell Res. 2008;18:733-44 pubmed publisher
immunocytochemistry; human western blot; human; fig 7a	Strauss G, Westhoff M, Fischer Posovszky P, Fulda S, Schanbacher M, Eckhoff S, et al. 4-hydroperoxy-cyclophosphamide mediates caspase-independent T-cell apoptosis involving oxidative stress-induced nuclear relocation of mitochondrial apoptogenic factors AIF and EndoG. Cell Death Differ. 2008;15:332-43 pubmed
	Teng B, Tam E, Benzie I, Siu P. Protective effect of caspase inhibition on compression-induced muscle damage. J Physiol. 2011;589:3349-69 pubmed publisher
	Zhang J, Ye J, Altafaj A, Cardona M, Bahi N, Llovera M, et al. EndoG links Bnip3-induced mitochondrial damage and caspase-independent DNA fragmentation in ischemic cardiomyocytes. PLoS ONE. 2011;6:e17998 pubmed publisher

product information

Catalog Number :

3035

Product Name :

EndoG Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

EndoG Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndooG also cleaves DNA in vitro.

ALTnames :

EndoG Antibody: Endonuclease G, mitochondrial, Endo G

Immunogen :

EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG.

Purity :

EndoG Antibody is affinity chromatography purified via peptide column.

Buffer :

EndoG Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC

Application Summary :

EndoG antibody can be used for detection of EndoG by Western blot at 2 g/mL. Antibody can also be used for immunohistochemistry starting at 15 g/mL.

Storage :

EndoG antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature. 2001;412(6842):95-9.

General References :

Parrish J, Li L, Klotz K, Ledwich D, Wang X, Xue D. Mitochondrial endonuclease G is important for apoptosis in C. elegans. Nature. 2001;412(6842):90-4

General References :

Hengartner MO. Apoptosis. DNA destroyers. Nature. 2001;412(6842):27, 29.

General References :

Widlak P, Li LY, Wang X, Garrard WT. Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. J Biol Chem. 2001;276(51):48404-9.

more info or order :

ProSci product webpage