Noxa Antibody | ProSci 2437 product information

product summary

company name :

ProSci

product type :

antibody

product name :

Noxa Antibody

catalog :

2437

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, western blot knockout validation

more info or order :

ProSci product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; fig 2	Bilardi R, Anstee N, Glaser S, Robati M, Vandenberg C, Cory S. Impact of loss of BH3-only proteins on the development and treatment of MLL-fusion gene-driven AML in mice. Cell Death Dis. 2016;7:e2351 pubmed publisher
western blot; human; fig 5	Long J, Schoonen P, Graczyk D, O Prey J, Ryan K. p73 engages A2B receptor signalling to prime cancer cells to chemotherapy-induced death. Oncogene. 2015;34:5152-62 pubmed publisher
western blot; human western blot; mouse western blot; rat	Sabirzhanov B, Zhao Z, Stoica B, Loane D, Wu J, Borroto C, et al. Downregulation of miR-23a and miR-27a following experimental traumatic brain injury induces neuronal cell death through activation of proapoptotic Bcl-2 proteins. J Neurosci. 2014;34:10055-71 pubmed publisher
western blot; mouse	O Brien T, Gorentla B, Xie D, Srivatsan S, McLeod I, He Y, et al. Regulation of T-cell survival and mitochondrial homeostasis by TSC1. Eur J Immunol. 2011;41:3361-70 pubmed publisher
	Rodust P, Fecker L, Stockfleth E, Eberle J. Activation of mitochondrial apoptosis pathways in cutaneous squamous cell carcinoma cells by diclofenac/hyaluronic acid is related to upregulation of Bad as well as downregulation of Mcl-1 and Bcl-w. Exp Dermatol. 2012;21:520-5 pubmed publisher

product information

Catalog Number :

2437

Product Name :

Noxa Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

Noxa Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified, which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9. Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53. Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development.

ALTnames :

Noxa Antibody: Noxa, Phorbol-12-myristate-13-acetate-induced protein 1

Immunogen :

Noxa antibody was raised against a synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa.

Purity :

Noxa Antibody is immunoaffinity chromatography purified IgG.

Buffer :

Noxa Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

Noxa antibody can be used for detection of Noxa by Western blot at at 0.5 - 2 g/mL. Antibody can also be used for immunohistochemistry starting at 1 g/mL. For immunofluorescence start at 10 g/mL.

Storage :

Noxa antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science 2000; 288:1053-8.

General References :

Nakano K and Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol. Cell 2001; 7:683-94.

General References :

Yu J, Zhang L, Hwang PM, et al. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol. Cell 2001; 7:673-82.

General References :

Eferl R, Ricci R, Kenner L, et al. Liver tumor development. c-Jun antagonizes the proapoptotic activity of p53. Cell 2003; 112:181-92.

more info or order :

ProSci product webpage