IKAP Antibody | ProSci 2337 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

IKAP Antibody

catalog :

2337

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry

product information

Catalog Number :

2337

Product Name :

IKAP Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse

Background :

IKAP Antibody: IKAP was initially identified as a scaffold protein of the I B kinase complex that could bind to IKK , IKK , NF- B, and the NF- B-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD). Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD. At least two isoforms of IKAP are known two exist.

ALTnames :

IKAP Antibody: FD, DYS, ELP1, IKAP, IKI3, TOT1, Elongator complex protein 1, IkappaB kinase complex-associated protein

Immunogen :

IKAP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human IKAP.

Purity :

IKAP Antibody is affinity chromatography purified via peptide column.

Buffer :

IKAP Antibody is supplied in PBS containing 0.02% sodium azide.

Uses :

E, WB, ICC, IF

Application Summary :

IKAP antibody can be used for detection of IKAP by Western blot at 0.5 to 1 g/mL. Antibody can also be used for immunocytochemistry starting at 1 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

IKAP antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Cohen L, Henzel WJ, and Baeuerle PA. IKAP is a scaffold protein of the IkB kinase complex. Nature 1998; 395:292-6.

General References :

Krappmann D, Hatada EN, Tegethoff S, et al. The I kappa B kinase (IKK) complex is tripartite and contains IKK gamma but not IKAP as a regular component. J. Biol. Chem.2000; 275:29779-87.

General References :

Anderson SL, Coli R, Daly IW, et al. Familial dysautonomia is caused by mutations of the IKAP gene. Am. J. Hum. Genet. 2001; 68:753-8.

General References :

Hawkes NA, Otero G, Winkler GS, et al. Purification and characterization of the human elongator complex. J. Biol. Chem.2002; 277:3047-52.