RIP3 Antibody | ProSci 2283 product information

product summary

company name :

ProSci

product type :

antibody

product name :

RIP3 Antibody

catalog :

2283

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation

more info or order :

ProSci product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 1 western blot knockout validation; mouse; fig 1 immunohistochemistry - paraffin section; mouse; fig 1	Wang S, Ni H, Dorko K, Kumer S, Schmitt T, Nawabi A, et al. Increased hepatic receptor interacting protein kinase 3 expression due to impaired proteasomal functions contributes to alcohol-induced steatosis and liver injury. Oncotarget. 2016;7:17681-98 pubmed publisher
western blot; mouse; loading ...; fig 1c	Moriwaki K, Chan F. Regulation of RIPK3- and RHIM-dependent Necroptosis by the Proteasome. J Biol Chem. 2016;291:5948-59 pubmed publisher
immunoprecipitation; mouse; fig 1 western blot; mouse; fig 4	Aaes T, Kaczmarek A, Delvaeye T, De Craene B, De Koker S, Heyndrickx L, et al. Vaccination with Necroptotic Cancer Cells Induces Efficient Anti-tumor Immunity. Cell Rep. 2016;15:274-87 pubmed publisher
western blot; mouse; loading ...; fig 2a	Shutinoski B, Alturki N, Rijal D, Bertin J, Gough P, Schlossmacher M, et al. K45A mutation of RIPK1 results in poor necroptosis and cytokine signaling in macrophages, which impacts inflammatory responses in vivo. Cell Death Differ. 2016;23:1628-37 pubmed publisher
western blot; mouse; fig 2d	Chen G, Cheng X, Zhao M, Lin S, Lu J, Kang J, et al. RIP1-dependent Bid cleavage mediates TNFα-induced but Caspase-3-independent cell death in L929 fibroblastoma cells. Apoptosis. 2015;20:92-109 pubmed publisher
western blot; mouse; fig 2	Dowling J, Nair A, Zhang J. A novel function of RIP1 in postnatal development and immune homeostasis by protecting against RIP3-dependent necroptosis and FADD-mediated apoptosis. Front Cell Dev Biol. 2015;3:12 pubmed publisher
western blot; mouse; fig 1	Wang X, Li Y, Liu S, Yu X, Li L, Shi C, et al. Direct activation of RIP3/MLKL-dependent necrosis by herpes simplex virus 1 (HSV-1) protein ICP6 triggers host antiviral defense. Proc Natl Acad Sci U S A. 2014;111:15438-43 pubmed publisher
western blot; mouse	McComb S, Shutinoski B, Thurston S, Cessford E, Kumar K, Sad S. Cathepsins limit macrophage necroptosis through cleavage of Rip1 kinase. J Immunol. 2014;192:5671-8 pubmed publisher
western blot; mouse	Liu S, Wang X, Li Y, Xu L, Yu X, Ge L, et al. Necroptosis mediates TNF-induced toxicity of hippocampal neurons. Biomed Res Int. 2014;2014:290182 pubmed publisher
western blot; mouse	Wu Y, Tan H, Huang Q, Sun X, Zhu X, Shen H. zVAD-induced necroptosis in L929 cells depends on autocrine production of TNFα mediated by the PKC-MAPKs-AP-1 pathway. Cell Death Differ. 2011;18:26-37 pubmed publisher
	Degterev A, Zhou W, Maki J, Yuan J. Assays for necroptosis and activity of RIP kinases. Methods Enzymol. 2014;545:1-33 pubmed publisher
	Arslan S, Scheidereit C. The prevalence of TNFα-induced necrosis over apoptosis is determined by TAK1-RIP1 interplay. PLoS ONE. 2011;6:e26069 pubmed publisher

product information

Catalog Number :

2283

Product Name :

RIP3 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Mouse, Rat

Background :

RIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF- B activation. Overexpression of RIP3 induces apoptosis and NF- B activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.

ALTnames :

RIP3 Antibody: Rip3, AW107945, 2610528K09Rik, Rip3, RIP-like protein kinase 3, RIP-3

Immunogen :

RIP3 antibody was raised against a 14 amino acid peptide near the carboxy terminus of murine RIP3.

Purity :

RIP3 Antibody is affinity chromatography purified via peptide column.

Buffer :

RIP3 Antibody is supplied in PBS containing 0.02% sodium azide.

Uses :

E, WB, IHC, IF

Application Summary :

RIP3 antibody can be used for detection of RIP3 by Western blot at 1 g/mL. An approximately 57 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

RIP3 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Yu PW, Huang BC, Shen M, Quast J, Chan E, Xu X, Nolan GP, Payan DG, Luo Y. Identification of RIP3, a RIP-like kinase that activates apoptosis and NF B. Curr Biol. 1999;9(10):539-42.

General References :

Sun X, Lee J, Navas T, Baldwin DT, Stewart TA, Dixit VM. RIP3, a novel apoptosis-inducing kinase. J Biol Chem. 1999;274(24):16871-5.

General References :

Pazdernik NJ, Donner DB, Goebl MG, Harrington MA. Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF- B. Mol Cell Bio. 1999; 19(10):6500-8 (WD0102)

more info or order :

ProSci product webpage