Nephrin Antibody | ProSci 2265 product information

product summary

company name :

ProSci

product type :

antibody

product name :

Nephrin Antibody

catalog :

2265

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

ProSci product webpage

citations: 1

Reference
Yun B, Lee S, Cho H, Kim J, Son G, Kim Y, et al. Expression of nephrin in the human placenta and fetal membranes. Mol Med Rep. 2015;12:5116-20 pubmed publisher

product information

Catalog Number :

2265

Product Name :

Nephrin Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

Nephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.

ALTnames :

Nephrin Antibody: CNF, NPHN, nephrin, Nephrin, Renal glomerulus-specific cell adhesion receptor

Immunogen :

Nephrin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Nephrin.

Purity :

Nephrin Antibody is affinity chromatography purified via peptide column.

Buffer :

Nephrin Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

Nephrin antibody can be used for detection of Nephrin by Western blot at 1 g/mL. Antibody can also be used for immunohistochemistry starting at 1 g/mL. For immunofluorescence start at 10 g/mL.

Storage :

Nephrin antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Kestila M, Lenkkeri U, Mannikko M, et al. Positionally cloned gene for a novel glomerular protein - Nephrin - is mutated in congenital nephrotic syndrome. Mol. Cell 1998; 1:575-582.

General References :

Tryggvason K. Unraveling the mechanisms of glomerular ultrafiltration: nephrin, a key component of the slit diaphragm. J. Am. Soc. Nephrol. 1999; 10:2440-5.

General References :

Tryggvason K and Wartiovaara J. Molecular basis of glomerular permselectivity. Curr, Opin. Nephrol. Hypertens. 2001; 10:543-9. (07-01D)

more info or order :

ProSci product webpage