ProSci

antibody

BACE Antibody

2253

0.02 mg, 0.1 mg

79, 315 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunocytochemistry

2253

BACE Antibody

Rabbit

Polyclonal

Unconjugated

Primary Antibody

0.02 mg, 0.1 mg

79, 315 USD

IgG

Human, Mouse

BACE Antibody: Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by two proteases. APP is first cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide, which is deposited in the brains of all suffers of Alzheimer's disease. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

BACE Antibody: ASP2, BACE, HSPC104, KIAA1149, Beta-secretase 1, Aspartyl protease 2, ASP2

BACE antibody was raised against a peptide corresponding to 17 amino acids at the carboxy terminus of human BACE.

BACE Antibody is Ion exchange chromatography purified.

BACE Antibody is supplied in PBS containing 0.02% sodium azide.

1 mg/mL

E, WB, ICC

BACE can be used for detection of BACE by Western blot at 1 g/mL. Antibody can also be used for immunocytochemistry starting at 10 g/mL.

BACE antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Vassar R, Bennett BD, Babu-Khan S, et al. -secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999;286:735-41

Hussain I, Powell D, Howlett DR , et al. Identification of a novel aspartic protease (Asp 2) as -secretase. Mol Cell Neurosci 1999;14:419-27

Yan R, Bienkowski MJ, Shuck ME, et al. Membrane-anchored aspartyl protease with Alzheimer's disease -secretase activity. Nature 1999;402:533-7

Sinha S, Anderson JP, Barbour R, et al. Purification and cloning of amyloid precursor protein -secretase from human brain. Nature 1999;402:537-40 (WD0500)