IRAK-2 Antibody | ProSci 2123 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

IRAK-2 Antibody

catalog :

2123

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

product information

Catalog Number :

2123

Product Name :

IRAK-2 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human

Background :

IRAK-2 Antibody: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF- B, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF- B activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF- B activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.

ALTnames :

IRAK-2 Antibody: IRAK-2, Interleukin-1 receptor-associated kinase-like 2, IRAK-2

Immunogen :

IRAK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK2.

Specificity :

Anti-IRAK2 has no cross response to IRAK.

Purity :

IRAK-2 Antibody is affinity chromatography purified via peptide column.

Buffer :

IRAK-2 Antibody is supplied in PBS containing 0.02% sodium azide.

Uses :

E, WB, ICC, IF

Application Summary :

IRAK-2 antibody can be used for detection of IRAK2 by Western blot at 2 g/mL. A 65 kDa band should be detected. This polyclonal antibody can also detect IRAK2 by immunocytochemistry at 2 g/mL. For immunofluorescence start at 10 g/mL.

Storage :

IRAK-2 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997;278:1612-5.

General References :

Zhang FX, Kirschning CJ, Mancinelli R, Xu XP, Jin Y, Faure E, Mantovani A, Rothe M, Muzio M, Arditi M. Bacterial lipopolysaccharide activates nuclear factor- B through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes. J Biol Chem 1999;274:7611-4.

General References :

Yang RB, Mark MR, Gurney AL, Godowski PJ. Signaling events induced by lipopolysaccharide-activated toll-like receptor 2. J Immunol 1999;163:639-43. (RD1299)