IKK alpha Antibody | ProSci 2025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

IKK alpha Antibody

catalog :

2025

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

product information

Catalog Number :

2025

Product Name :

IKK alpha Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human

Background :

IKK alpha Antibody: Nuclear factor kappa B (NF- B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF- B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF- B is associated with I B proteins in the cell cytoplasm, which inhibit NF- B activity. The long-sought I B kinase (IKK), which phosphorylates I B, and mediates I B degradation and NF- B activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKK and IKK ). IKK and IKK interact with each other and both are essential for the NF- B activation. IKK specifically phosphorylates IkB-alpha. IKK is expressed in variety of human tissues.

ALTnames :

IKK alpha Antibody: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase alpha

Immunogen :

IKK alpha antibody was raised against a 19 amino acid peptide near the carboxy terminus of human IKK alpha.

Specificity :

Antibody has no cross response to IKKb or IKKg.

Purity :

IKK alpha Antibody is affinity chromatography purified via peptide column.

Buffer :

IKK alpha Antibody is supplied in PBS containing 0.02% sodium azide.

Uses :

E, WB, ICC, IF

Application Summary :

IKK alpha can be used for detection of IKK alpha by Western blot at 1 g/mL. An 85 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 1 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

IKK alpha antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive I B kinase that activates the transcription factor NF- B. Nature 1997;388:548-54

General References :

Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an I B kinase. Cell 1997;90:373-83

General References :

Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The I B kinase complex (IKK) contains two kinase subunits, IKK and IKK , necessary for I B phosphorylation and NF- B activation. Cell 1997;91:243-52

General References :

Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. I B kinase- : NF- B activation and complex formation with I B kinase- and NIK. Science 1997;278:866-9