Apaf1 Antibody | ProSci 2013 product information

product summary

company name :

ProSci

product type :

antibody

product name :

Apaf1 Antibody

catalog :

2013

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

ProSci product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human	Shinoura N, Sakurai S, Asai A, Kirino T, Hamada H. Over-expression of APAF-1 and caspase-9 augments radiation-induced apoptosis in U-373MG glioma cells. Int J Cancer. 2001;93:252-61 pubmed
immunohistochemistry - paraffin section; human	Leo C, Richter C, Horn L, Schutz A, Pilch H, Hockel M. Expression of Apaf-1 in cervical cancer correlates with lymph node metastasis but not with intratumoral hypoxia. Gynecol Oncol. 2005;97:602-6 pubmed
western blot; human	Shinoura N, Sakurai S, Asai A, Kirino T, Hamada H. Co-transduction of Apaf-1 and caspase-9 augments etoposide-induced apoptosis in U-373MG glioma cells. Jpn J Cancer Res. 2001;92:467-74 pubmed
western blot; human	Shinoura N, Sakurai S, Shibasaki F, Asai A, Kirino T, Hamada H. Co-transduction of Apaf-1 and caspase-9 highly enhances p53-mediated apoptosis in gliomas. Br J Cancer. 2002;86:587-95 pubmed
	Chang L, Kreko-Pierce T, Eaton B. The guanine exchange factor Gartenzwerg and the small GTPase Arl1 function in the same pathway with Arfaptin during synapse growth. Biol Open. 2015;4:947-53 pubmed publisher
	Pfeifer L, Adams G, Pierson R, Singh J. Ultrasound biomicroscopy: a non-invasive approach for in vivo evaluation of oocytes and small antral follicles in mammals. Reprod Fertil Dev. 2013;26:48-54 pubmed publisher
	Macpherson R, Vandenboom R, Roy B, Peters S. Skeletal muscle PLIN3 and PLIN5 are serine phosphorylated at rest and following lipolysis during adrenergic or contractile stimulation. Physiol Rep. 2013;1:e00084 pubmed publisher

product information

Catalog Number :

2013

Product Name :

Apaf1 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

Apaf1 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.

ALTnames :

Apaf1 Antibody: CED4, APAF-1, KIAA0413, Apoptotic protease-activating factor 1

Immunogen :

Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse.

Purity :

Apaf1 Antibody is affinity chromatography purified via peptide column.

Buffer :

Apaf1 Antibody is supplied in PBS containing 0.02% sodium azide.

Uses :

E, WB, IHC, IF

Application Summary :

Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 g/mL. A 115 - 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 g/mL. For immunofluorescence start at 10 g/mL.

Storage :

Apaf1 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13

General References :

Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998;94:727-37

General References :

Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation ofApaf1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89

General References :

Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts with Apaf1 and inhibits Apaf1-dependent caspase-9 activation. Proc Natl Acad Sci USA 1998;95:4386-91 (RD1299)

more info or order :

ProSci product webpage