ICAD Antibody | ProSci 2003 product information

product summary

company name :

ProSci

product type :

antibody

product name :

ICAD Antibody

catalog :

2003

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, ELISA

more info or order :

ProSci product webpage

citations: 4

Reference
Zhang X, Alber S, Watkins S, Kochanek P, Marion D, Graham S, et al. Proteolysis consistent with activation of caspase-7 after severe traumatic brain injury in humans. J Neurotrauma. 2006;23:1583-90 pubmed
Hu H, Sa Q, Koehler T, Aronson A, Zhou D. Inactivation of Bacillus anthracis spores in murine primary macrophages. Cell Microbiol. 2006;8:1634-42 pubmed
Gillan L, Kroetsch T, Maxwell W, Evans G. Assessment of in vitro sperm characteristics in relation to fertility in dairy bulls. Anim Reprod Sci. 2008;103:201-14 pubmed
Warner A, Chakrabortee S, Tunnacliffe A, Clegg J. Complexity of the heat-soluble LEA proteome in Artemia species. Comp Biochem Physiol Part D Genomics Proteomics. 2012;7:260-7 pubmed publisher

product information

Catalog Number :

2003

Product Name :

ICAD Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Mouse

Background :

ICAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.

ALTnames :

ICAD Antibody: ICAD, DFF35, Dff45, ICAD-L, ICAD-S, A330085O09Rik, Icad, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, DFF-45

Immunogen :

ICAD antibody was raised against a 20 amino acid peptide near the carboxy terminus of mouse ICAD.

Purity :

ICAD Antibody is affinity chromatography purified via peptide column.

Buffer :

ICAD Antibody is supplied in PBS containing 0.02% sodium azide.

Uses :

E, WB

Application Summary :

ICAD antibody can be used for detection of of ICAD by Western blot at 1:1000 dilution. A 45 kDa band can be detected.

Storage :

ICAD antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184

General References :

Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50

General References :

Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99

General References :

Wyllie A. Apoptosis. An endonuclease at last. Nature 1998;39120-21 (RD1299)

more info or order :

ProSci product webpage