ICAD Antibody | ProSci 2001 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

ICAD Antibody

catalog :

2001

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

product information

Catalog Number :

2001

Product Name :

ICAD Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Mouse

Background :

ICAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which is cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.

ALTnames :

ICAD Antibody: ICAD, DFF35, Dff45, ICAD-L, ICAD-S, A330085O09Rik, Icad, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, DFF-45

Immunogen :

ICAD antibody was raised against a peptide corresponding to amino acids near the amino terminus of mouse ICAD.

Purity :

ICAD Antibody is affinity chromatography purified via peptide column.

Buffer :

ICAD Antibody is supplied in PBS containing 0.02% sodium azide.

Uses :

E, WB, IHC, IF

Application Summary :

ICAD antibody can be used for detection of of ICAD by Western blot at 1 g/mL. A 45 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 g/mL. For immunofluorescence start at 10 g/mL.

Storage :

ICAD antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184

General References :

Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50

General References :

Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99

General References :

Wyllie A. Apoptosis. An endonuclease at last. Nature 1998;39120-21 (RD1299)