FLIP Antibody | ProSci 1161 product information

product summary

company name :

ProSci

product type :

antibody

product name :

FLIP Antibody

catalog :

1161

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry

more info or order :

ProSci product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1 ug/ml	Bucur O, Pennarun B, Stancu A, Nadler M, Muraru M, Bertomeu T, et al. Poor antibody validation is a challenge in biomedical research: a case study for detection of c-FLIP. Apoptosis. 2013;18:1154-62 pubmed publisher
	Frese S, Brunner T, Gugger M, Uduehi A, Schmid R. Enhancement of Apo2L/TRAIL (tumor necrosis factor-related apoptosis-inducing ligand)-induced apoptosis in non-small cell lung cancer cell lines by chemotherapeutic agents without correlation to the expression level of cellular protease caspase-8 inhibitor. J Thorac Cardiovasc Surg. 2002;123:168-74 pubmed

product information

Catalog Number :

1161

Product Name :

FLIP Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

FLIP Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.

ALTnames :

FLIP Antibody: CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, CASH, CASP8 and FADD-like apoptosis regulator, Caspase homolog

Immunogen :

FLIP antibody was raised against a peptide corresponding to amino acids near the C-terminus of human FLIPaFLIPl form.

Specificity :

Antibody recognizes the FLIPa only.

Purity :

FLIP Antibody is Antibody is DEAE purified.

Buffer :

FLIP Antibody is supplied in PBS containing 0.02% sodium azide.

Uses :

E, WB, ICC, IF

Application Summary :

Flip antibody can be used for detection of FLIP by Western blot at 1 g/mL. A 55 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 g/mL. For immunofluorescence start at 10 g/mL.

Storage :

FLIP antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Thome M, Schneider P, Hofmann K, et al. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997;386:517-521

General References :

Irmler M, Thome M, Hahne M, et al. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195

General References :

Shu HB, Halpin DR , Goeddel DV. Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 1997;6:751-763

General References :

Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem 1997;272:17255-17257

more info or order :

ProSci product webpage