DR4 Antibody | ProSci 1139 product information

product summary

company name :

ProSci

product type :

antibody

product name :

DR4 Antibody

catalog :

1139

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - free floating section

more info or order :

ProSci product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 2a	Lafont E, Kantari Mimoun C, Dráber P, De Miguel D, Hartwig T, Reichert M, et al. The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cellÂ death.. EMBO J. 2017;36:1147-1166 pubmed publisher
western blot; human; fig 6	Ashley D, Riffkin C, Lovric M, Mikeska T, Dobrovic A, Maxwell J, et al. In vitro sensitivity testing of minimally passaged and uncultured gliomas with TRAIL and/or chemotherapy drugs. Br J Cancer. 2008;99:294-304 pubmed publisher
western blot; human	Lonergan M, Aponso D, Marvin K, Helliwell R, Sato T, Mitchell M, et al. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), TRAIL receptors, and the soluble receptor osteoprotegerin in human gestational membranes and amniotic fluid during pregnancy and labor at term and preterm. J Clin Endocrinol Metab. 2003;88:3835-44 pubmed
western blot; human; 1:500	Malhi H, Barreyro F, Isomoto H, Bronk S, Gores G. Free fatty acids sensitise hepatocytes to TRAIL mediated cytotoxicity. Gut. 2007;56:1124-31 pubmed
immunohistochemistry - free floating section; mouse	Bäurle J, Frischmuth S, Kranda K. TRAIL-related death receptors in normal, Lurcher and weaver mutant mouse brain. Neurosci Lett. 2004;372:46-51 pubmed
flow cytometry; human immunocytochemistry; human western blot; human	Linderoth E, Pilia G, Mahajan N, Ferby I. Activated Cdc42-associated kinase 1 (Ack1) is required for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor recruitment to lipid rafts and induction of cell death. J Biol Chem. 2013;288:32922-31 pubmed publisher
	Hartwig T, Montinaro A, von Karstedt S, Sevko A, Surinova S, Chakravarthy A, et al. The TRAIL-Induced Cancer Secretome Promotes a Tumor-Supportive Immune Microenvironment via CCR2. Mol Cell. 2017;65:730-742.e5 pubmed publisher
	Perimenis P, Galaris A, Voulgari A, Prassa M, Pintzas A. IAP antagonists Birinapant and AT-406 efficiently synergise with either TRAIL, BRAF, or BCL-2 inhibitors to sensitise BRAFV600E colorectal tumour cells to apoptosis. BMC Cancer. 2016;16:624 pubmed publisher
	Drosopoulos K, Roberts M, Cermak L, Sasazuki T, Shirasawa S, Andera L, et al. Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway. J Biol Chem. 2005;280:22856-67 pubmed

product information

Catalog Number :

1139

Product Name :

DR4 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human

Background :

DR4 Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4). The ligand for this novel death receptor has been identified and termed TRAIL2, 3, which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1). DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF- B activation in many tissues and cells.

ALTnames :

DR4 Antibody: DR4, APO2, CD261, TRAILR1, TRAILR-1, DR4, Tumor necrosis factor receptor superfamily member 10A, Death receptor 4, TRAIL receptor 1

Immunogen :

DR4 antibody was raised against a peptide corresponding to 19 amino acids near the carboxy terminus of human DR4 protein.

Purity :

DR4 Antibody is Antibody is affinity chromatography purified via peptide column.

Buffer :

DR4 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, ICC

Application Summary :

DR4 antibody can be used for detection of DR4 expression by Western blot at 0.5 g/mL. Antibody can also be used for immunocytochemistry starting at 10 g/mL.

Storage :

DR4 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner R; Ni J; Dixit VM. The receptor for the cytotoxic ligand TRAIL. Science; 1997;276:111-113

General References :

Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl JK, Sutherland GR, Smith TD, Rauch C, Smith CA, et al. Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity 1995;3:673-682

General References :

Pitti RM; Marsters SA; Ruppert S; Donahue CJ; Moore A; Ashkenazi A. Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J. Biol. Chem. 1996;271:12687-90

General References :

Schneider P, Thome M, Burns K, Bodmer JL, Hofmann K, Kataoka T, Holler N, Tschopp J. TRAIL receptors 1 (DR4) and 2 (DR5) signal FADD-dependent apoptosis and activate NF- B. Immunity 1997;7:831-836 (RD1299)

more info or order :

ProSci product webpage