antibody

Mouse Monoclonal Antibody to HLA-B

31114

0.1 mg

monoclonal

mouse

nonconjugated

2G7B10

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

Mouse Monoclonal Antibody to HLA-B

Human;

2G7B10;

Mouse IgG1

0.1 mg

4℃; -20℃ for long term storage

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.;

Purified recombinant fragment of human HLA-B (AA: 241-362) expressed in E. Coli.

Purified antibody in PBS with 0.05% sodium azide

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400

1.Pharmacogenomics J. 2015 Oct;15(5):467-72. ; 2.J Immunol. 2014 Jun 1;192(11):4967-76.;

Monoclonal Antibody

58.8kDa

MI; WS2; CMM8; WS2A; bHLHe32