antibody

Mouse Monoclonal Antibody to CCNE1

30675

0.1 mg

monoclonal

mouse

nonconjugated

5F8C5

The same clone is also sold as:

• Abnova: MAB12242
• Novus Biologicals: NBP2-37247
• Abcam: ab211342

human, mouse

western blot, ELISA, flow cytometry

Mouse Monoclonal Antibody to CCNE1

Human; Mouse;

5F8C5;

Mouse IgG1

0.1 mg

4℃; -20℃ for long term storage

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.

Purified recombinant fragment of human CCNE1 (AA: 307-410) expressed in E. Coli.

Purified antibody in PBS with 0.05% sodium azide.

ELISA: 1/10000; WB: 1/500 - 1/2000; FCM: 1/200 - 1/400

1. Cancer Res. 2010 Jun 15;70(12):5074-84. ; 2. Cancer. 2010 Jun 1;116(11):2621-34.

Monoclonal Antibody

47kDa

CCNE