Mouse Monoclonal Antibody to INCENP | ProMab 30388 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to INCENP

catalog :

30388

quantity :

0.1 mg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3D2

The same clone is also sold as:

Novus Biologicals: NBP2-37471
Invitrogen: MA5-17100

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

product information

Catalog :

30388

Title :

Mouse Monoclonal Antibody to INCENP

Species :

Human

Clone :

3D2;

lsotype :

Mouse IgG1

Size :

0.1 mg

Storage :

4℃; -20℃ for long term storage

Description :

In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis

Immunogen :

Purified recombinant fragment of human INCENP (AA: 369-583) expressed in E. Coli. ;

Formulation :

Purified antibody in PBS with 0.05% sodium azide

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400

References :

1.J Cell Biol. 2009 Nov 30;187(5):637-53. ; 2.Genes Cells. 2011 Jun;16(6):652-69. ;

Type :

Monoclonal Antibody

MW :

105.4kDa