Mouse Monoclonal Antibody to SHH | ProMab 30375 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to SHH

catalog :

30375

quantity :

0.1 mg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5H4

The same clone is also sold as:

Novus Biologicals: NBP2-22126
Abcam: ab135240
Invitrogen: MA5-17173
OriGene: TA336923

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, flow cytometry

product information

Catalog :

30375

Title :

Mouse Monoclonal Antibody to SHH

Species :

Human; Mouse; Monkey

Clone :

5H4;

lsotype :

Mouse IgG1

Size :

0.1 mg

Storage :

4℃; -20℃ for long term storage

Description :

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.

Immunogen :

Purified recombinant fragment of human SHH (AA: 26-161) expressed in E. Coli. ; ;

Formulation :

Purified antibody in PBS with 0.05% sodium azide

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400

References :

1.Br J Cancer. 2010 Feb 16;102(4):738-47. ; 2.Mol Cancer. 2009 Dec 16;8:123. ;

Type :

Monoclonal Antibody

MW :

49.6kDa

Aliases :

TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5