Mouse Monoclonal Antibody to NEDD8 | ProMab 30314 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to NEDD8

catalog :

30314

quantity :

0.1 mg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1.A.7

The same clone is also sold as:

Abnova: MAB10825
Novus Biologicals: NBP2-37524
OriGene: AM06718PU-N
Invitrogen: MA5-17133

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

product information

Catalog :

30314

Title :

Mouse Monoclonal Antibody to NEDD8

Species :

Human

Clone :

1A7;

lsotype :

Mouse IgG1

Size :

0.1 mg

Storage :

4℃; -20℃ for long term storage

Description :

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.Tissue specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.

Immunogen :

Purified recombinant fragment of human NEDD8 expressed in E. Coli. ;

Formulation :

Purified antibody in PBS with 0.05% sodium azide

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400

References :

1. Cell. 2009 Jul 23;138(2):389-403. ; 2. Biochem Biophys Res Commun. 2009 Apr 10;381(3):443-7. ;

Type :

Monoclonal Antibody

MW :

9kDa

Aliases :

NEDD-8