Mouse Monoclonal Antibody to SMAD2 | ProMab 30242 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to SMAD2

catalog :

30242

quantity :

100μl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5G7

The same clone is also sold as:

Invitrogen: MA5-15877
OriGene: AM06653SU-N
Abnova: MAB10391
Abcam: ab119907

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

product information

Catalog :

30242

Title :

Mouse Monoclonal Antibody to SMAD2

Species :

Human

Clone :

5G7;

lsotype :

Mouse IgG1

Size :

100μl

Storage :

4℃; -20℃ for long term storage

Description :

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.

Immunogen :

Purified recombinant fragment of human SMAD2 expressed in E. Coli. ;

Formulation :

Ascitic fluid containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400

References :

1. J Biol Chem. 2009 Dec 4;284(49):34145-56. ; 2. Cloning Stem Cells. 2009 Sep;11(3):427-35.

Type :

Monoclonal Antibody

MW :

52kDa

Aliases :

JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; MGC22139; MGC34440