Mouse Monoclonal Antibody to CRTC2 | ProMab 30120 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to CRTC2

catalog :

30120

quantity :

100μl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5B10

The same clone is also sold as:

Invitrogen: MA5-15710
Abnova: MAB10615
OriGene: AM06534SU-N

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

product information

Catalog :

30120

Title :

Mouse Monoclonal Antibody to CRTC2

Species :

Human; Monkey

Clone :

5B10;

lsotype :

Mouse IgG1

Size :

100μl

Storage :

4℃; -20℃ for long term storage

Description :

Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic β-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes β-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling . The protein Torc2 (transducer of regulated CREB activity 2) functions as a CREB co-activator and is implicated in mediating the effects of these two pathways . In quiescent cells, Torc2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its dissociation from 14-3-3 proteins. Dephosphorylated Torc2 enters the nucleus to promote CREB-dependent transcription. Torc2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting.Tissue specificity: Most abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas .

Immunogen :

Purified recombinant fragment of human CRTC2 expressed in E. Coli.

Formulation :

Ascitic fluid containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400

References :

1. Mol Syst Biol. 2007;3:89. ; 2. Nature. 2007 Sep 20;449(7160):366-9. ; 3. J Biol Chem. 2009 Mar 20;284(12):8033-41.

Type :

Monoclonal Antibody

MW :

80kDa

Aliases :

TORC2; TORC-2; CRTC2