Mouse Monoclonal Antibody to SIRT1 | ProMab 30086 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to SIRT1

catalog :

30086

quantity :

100μl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

#1F3

The same clone is also sold as:

Invitrogen: MA5-15677
Novus Biologicals: NBP1-51641, NBP2-80949
Abcam: ab104833
MyBioSource: MBS850730
Abnova: MAB10318
Cell Signaling Technology: 8469
OriGene: AM06495SU-N, TA336801

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

product information

Catalog :

30086

Title :

Mouse Monoclonal Antibody to SIRT1

Species :

Human;

Clone :

1F3;

lsotype :

Mouse IgG1

Size :

100μl

Storage :

4℃; -20℃ for long term storage

Description :

The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53.SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.

Immunogen :

Purified recombinant fragment of human SIRT1 expressed in E. Coli.

Formulation :

Ascitic fluid containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400

References :

1. Clin Cancer Res. 2009 Jul 1;15(13):4453-9. ; 2. Cell. 2009 Jul 23;138(2):389-403. ; 3. J Biol Chem. 2009 Oct 16;284(42):28762-74.

Type :

Monoclonal Antibody

MW :

120kDa

Aliases :

SIR2L1; SIRT1