Mouse Monoclonal Antibody to CDC27 | ProMab 30064 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to CDC27

catalog :

30064

quantity :

100μl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5C12

The same clone is also sold as:

Invitrogen: MA5-15655
OriGene: AM06470SU-N
Abnova: MAB10545

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

product information

Catalog :

30064

Title :

Mouse Monoclonal Antibody to CDC27

Species :

Human

Clone :

5C12;

lsotype :

Mouse IgG1

Size :

100μl

Storage :

4℃; -20℃ for long term storage

Description :

Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.

Immunogen :

Purified recombinant fragment of human CDC27 expressed in E. Coli. ;

Formulation :

Ascitic fluid containing 0.03% sodium azide. ;

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000

References :

1. Mol Cell Biol. 2004 May;24(9):3577-87. ; 2. J Biol Chem. 2004 Oct 1;279(40):42128-38.

Type :

Monoclonal Antibody

MW :

91kDa

Aliases :

APC3; HNUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E;