Mouse Monoclonal Antibody to SHH | ProMab 30056 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to SHH

catalog :

30056

quantity :

100μl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8G3

The same clone is also sold as:

OriGene: AM06459SU-N
Abnova: MAB16325, MAB10317
Invitrogen: MA5-15647

reactivity :

human

application :

western blot, ELISA

product information

Catalog :

30056

Title :

Mouse Monoclonal Antibody to SHH

Species :

Human

Clone :

8G3;

lsotype :

Mouse IgG1

Size :

100μl

Storage :

4℃; -20℃ for long term storage

Description :

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.

Immunogen :

Purified recombinant fragment of human SHH expressed in E. Coli.

Formulation :

Ascitic fluid containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000

References :

1. Cancer Lett. 2010 Jan 1;287(1):44-53. ; 2. Oncogene. 2009 Oct 8;28(40):3513-25. ; 3. J Biol Chem. 2009 Nov 20;284(47):32562-71.

Type :

Monoclonal Antibody

MW :

49.6kDa

Aliases :

TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5; SHH