Mouse Monoclonal Antibody to CDC2 | ProMab 30040 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to CDC2

catalog :

30040

quantity :

100μl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8C5A7F10

The same clone is also sold as:

OriGene: AM06438SU-N
Abnova: MAB10544
Invitrogen: MA5-15631

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, flow cytometry

product information

Catalog :

30040

Title :

Mouse Monoclonal Antibody to CDC2

Species :

Human

Clone :

8C5A7F10;

lsotype :

Mouse IgG1

Size :

100μl

Storage :

4℃; -20℃ for long term storage

Description :

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Immunogen :

Purified recombinant fragment of CDC2 expressed in E. Coli.

Formulation :

Ascitic fluid containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400

References :

1. Mol Biol Cell. 2008 Aug;19(8):3536-43. ; 2. Eur J Cancer. 2009 May;45(8):1466-73.

Type :

Monoclonal Antibody

MW :

34kDa

Aliases :

CDC2; CDC28A; P34CDC2; MGC111195; DKFZp686L20222; CDK1