antibody

Mouse Monoclonal Antibody to RAG2

30031

100μl

monoclonal

mouse

nonconjugated

4D5

The same clone is also sold as:

• OriGene: AM06429SU-N
• Invitrogen: MA5-15622
• Abnova: MAB10311

western blot, ELISA

Mouse Monoclonal Antibody to RAG2

Human

4D5;

Mouse IgG1

100μl

4℃; -20℃ for long term storage

This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

Purified recombinant fragment of human RAG2(350-527aa) expressed in E. Coli.

Ascitic fluid containing 0.03% sodium azide. ;

ELISA: 1/10000; WB: 1/500 - 1/2000

1. J Biol Chem. 2004 Sep 10;279(37):38360-8. ; 2. Immunity. 2005 Aug;23(2):203-12. ; 3. J Clin Invest. 2010 Apr 1;120(4):1337-44. doi: 10.1172/JCI41305.

Monoclonal Antibody

59kDa

RAG-2; RAG2