Mouse Monoclonal Antibody to p44/42 MAPK (Erk1/2) | ProMab 30014 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to p44/42 MAPK (Erk1/2)

catalog :

30014

quantity :

100μl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

#3F8

The same clone is also sold as:

Abnova: MAB10379

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, flow cytometry

product information

Catalog :

30014

Title :

Mouse Monoclonal Antibody to p44/42 MAPK (Erk1/2)

Species :

Human; Mouse

Clone :

3F8;

lsotype :

Mouse IgG2b

Size :

100μl

Storage :

4℃; -20℃ for long term storage

Description :

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors such as U0126 and PD98059.

Immunogen :

Purified recombinant fragment of human MAPK expressed in E. Coli.

Formulation :

Ascitic fluid containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400

References :

1. FEBS Lett. 1992 Jun 15;304(2-3):170-8. ; 2. Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5361-5. ; 3. J Biol Chem. 2009 Nov 27;284(48):33456-65. ; 4. BMC Biol. 2009 Oct 27;7:70. ;

Type :

Monoclonal Antibody

MW :

42kDa

Aliases :

ERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; MAPK1