Mouse Monoclonal Antibody to BRAF | ProMab 20323 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to BRAF

catalog :

20323

quantity :

100μl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

'1H12

The same clone is also sold as:

Novus Biologicals: NBP1-47433
Abnova: MAB10422
Invitrogen: MA5-15495
OriGene: AM06317SU-N

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

product information

Catalog :

20323

Title :

Mouse Monoclonal Antibody to BRAF

Species :

Human, Mouse

Clone :

1H12;

lsotype :

Mouse IgG1

Size :

100μl

Storage :

4℃; -20℃ for long term storage

Description :

BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1; RAFB1; B-RAF1; FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.

Immunogen :

Purified recombinant fragment of human BRAF expressed in E. Coli. ;

Formulation :

Ascitic fluid containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000

References :

1. J Clin Oncol. 2008 Dec 10;26(35):5705-12. ; 2. Endocr Relat Cancer. 2006 Mar;13(1):257-69.

Type :

Monoclonal Antibody

MW :

87kDa

Aliases :

BRAF1; RAFB1; B-RAF1; FLJ95109