Mouse Monoclonal Antibody to MLH1 | ProMab 20246 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to MLH1

catalog :

20246

quantity :

100μl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4C9C7

The same clone is also sold as:

Abnova: MAB15272
Invitrogen: MA5-15431
Cell Signaling Technology: 3515
OriGene: AM06257SU-N
MyBioSource: MBS245337

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

product information

Catalog :

20246

Title :

Mouse Monoclonal Antibody to MLH1

Species :

Human; Monkey

Clone :

4C9C7;

lsotype :

Mouse IgG1

Size :

100μl

Storage :

4℃; -20℃ for long term storage

Description :

DNA-mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. Lack of a functional DNA-mismatch repair pathway is a common characteristic of several different types of human cancers, either due to an MMR gene mutation or promoter-methylation gene silencing. MLH1 is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in hereditary nonpolyposis colon cancer (HNPCC). MLH1 is an integral part of the protein complex responsible for mismatch repair expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder, and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Inactivation of the MLH1 gene causes genome instability and predisposition to cancer. MLH1 also plays a role in meiotic recombination.

Immunogen :

Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli. ;

Formulation :

Ascitic fluid containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000

References :

1. Int J Cancer. 2007 Aug 1;121(3):555-8. ; 2. Autophagy. 2007 Jul-Aug;3(4):368-70. ; 3. Fam Cancer. 2008 Jun;7(2):163-172.

Type :

Monoclonal Antibody

MW :

85kDa

Aliases :

FCC2; COCA2; HNPCC