Mouse Monoclonal Antibody to CHK2 | ProMab 20223 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to CHK2

catalog :

20223

quantity :

100μl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1C12B8

The same clone is also sold as:

OriGene: AM06242SU-N
Novus Biologicals: NBP1-47549-0.025ml
Invitrogen: MA5-15416
Abnova: MAB15266

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

product information

Catalog :

20223

Title :

Mouse Monoclonal Antibody to CHK2

Species :

Human

Clone :

1C12B8;

lsotype :

Mouse IgG2b

Size :

100μl

Storage :

4℃; -20℃ for long term storage

Description :

CHK2: CHK2 checkpoint homolog (S. pombe). In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

Immunogen :

Purified recombinant fragment of human CHK2 (aa481-531) expressed in E. Coli. ;

Formulation :

Ascitic fluid containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000

References :

1. Int J Cancer. 2007 Dec 15;121(12):2661-7. ; 2. Nat Rev Cancer. 2007 Dec;7(12):925-36. ; 3. Carcinogenesis. 2008 Apr;29(4):762-5.

Type :

Monoclonal Antibody

MW :

61kDa

Aliases :

CDS1; LFS2; CHEK2