Mouse Monoclonal Antibody to Calreticulin | ProMab 20177 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to Calreticulin

catalog :

20177

quantity :

100µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

N/A

reactivity :

human

application :

western blot, ELISA

product information

Catalog :

20177

Title :

Mouse Monoclonal Antibody to Calreticulin

Species :

Human;

Clone :

N/A;

lsotype :

Size :

100µl

Storage :

4?; -20? for long term storage

Description :

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

Immunogen :

Synthetic peptide corresponding to aa(E-E-E-D-V-P-G-Q-A-K-D-E-L-C)of human Calreticulin, conjugated to KLH.

Formulation :

Rabbit anti-serum.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000

References :

1. J Biol Chem. 2006 May 5;281(18):12841-8. Epub 2006 Mar 9. ; 2. Biochim Biophys Acta. 2006 May;1760(5):745-53. Epub 2006 Feb 28. ; 3. Oncol Rep. 2007 May;17(5):1101-7. ;

Type :

Monoclonal Antibody

Aliases :

RO; CRT; SSA; cC1qR; FLJ26680; CALR